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. 2004 Feb 9;101(7):2064–2069. doi: 10.1073/pnas.0308434100

Fig. 2.

Fig. 2.

Immunolocalization of WNK4 in kidney, pancreas, liver, and epididymis. Frozen sections of mouse kidney, pancreas, liver, and epididymis were stained with anti-WNK4 (red) and anti-ZO-1 (green) and analyzed by immunofluorescence microscopy. Composite images and views for WNK4 and ZO-1 alone are shown. (a) A renal tubule in longitudinal section is shown; WNK4 is confined to tubule epithelium and in this nephron segment largely colocalizes with the TJ protein ZO-1. (b) A large pancreatic duct in longitudinal section is shown; WNK4 expression is confined to pancreatic duct epithelia and largely overlaps with ZO-1. (c) A bile duct in cross section demonstrates that WNK4 localizes to epithelial cells that line the biliary tract lumen (L). WNK4 localization largely overlaps with ZO-1. (d) Image of the epididymis in cross section demonstrates WNK4 expression in the columnar epithelial cells lining the epididymal lumen (L) as well as more basal layers. WNK4 becomes more tightly membrane associated with apical progression. (Magnifications: ×600.)