Figure 3. (A) Distributions of AQUA scores for Lin28 (top) and Oct4 (bottom) expressions in the Yale Ovarian Cancer Cohort. The range, mean and median values are indicated. (B) Examples of immunofluorescence of human epithelial ovarian cancer samples using antibodies specific for Lin28 (I, in red), Oct4 (V, in red) or cytokeratin (II and VI, in green), respectively. The Dapi stain (III and VII, in blue) indicates cell nuclear compartment. III, merge of cytokeratin and Dapi; VII, merge of Oct4 and Dapi; IV, merge of cytokeratin and Lin28; VIII, merge of cytokeratin and Oct4. Bar = 20 μm. (C) Survival curves from a Cox proportional hazards regression analysis that included as covariates Lin28 and Oct4, along with their interaction term. Assessment of Lin28 and Oct4 expressions was performed in 3-fold redundancy.