Figure 1. MicroRNA profiling and validation in MCF7 cells in response to CDK4/6 inhibitor. (A) Heat map of differentially regulated microRNAs. (B) Graphic representation of differentially expressed microRNAs in response to DMSO or PD 0332991. (C) qRT-PCR validation of miR 25, 93 and 106b. (D) In situ hybridization detection of miR-25. (E) In situ hybridization detection of miR-106b. Each data point is a mean ± SD from three or more independent experiments. p < 0.05 were considered as significant.