Figure 7. RNA immunoprecipitation, p21, PTEN mRNA and miR106b-cluster analysis in response to CDK4/6 inhibitor in MCF7 cells. (A) Immunoprecipitation of Ago2, western blot of Ago2 and loading control lamin b. (B) qRT-PCR signals of Ago2 bound p21Cip1/Waf1 and PTEN/MMAC1, GAPDH mRNA in response to DMSO or PD 0332991. (C) qRT-PCR signals of Ago2-bound miR25. (D) qRT-PCR signals of Ago2-bound miR93. (E) qRT-PCR signals of Ago2-bound mi106b, and the signals were normalized to U6 internal control. Each data point is a mean ± SD from three or more independent experiments. p < 0.05 were considered as significant.