Figure 8. Functional analysis of miR106b-cluster in MCF7 cells in response to CDK4/6 inhibitor. (A) Flow cytometric analysis of BrdU uptake in MCF7 cells expressing miR106b-cluster (left panel), and QRT-PCR analysis of p21 and PTEN mRNA levels in MCF7 control and cells expressing mir106B-cluster in response PD 0332991 right panel. (B) Wound-healing assay. (C) Cell migration assay/invasion. (D) Quantitation of cell migration/invasion assay. Each data point is a mean ± SD from three or more independent experiments. p < 0.05 were considered as significant.