Figure 2. Flow sorting strategy for the day 13 and day 20 differentiated human embryonic stem cells.

Cells were sorted according to their expression of CD41 and CD34 on day (d)13 and CD41, CD34 and CD45 on d20. (A) Gating strategy used to sort 4 fractions on d 13 and (B) 5 fractions on d20. Cells that were CD34⁻ CD45⁻ at d 20 were also CD41⁻. The percent distribution (mean±SD) for each sorted fraction is shown (n = 5).