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A decrease in islet damage was observed in different assays after islet isolation supplemented with 10 mM GEE. (A) Membrane integrity evaluated with Syto Green/Ethidium Bromide (P<0.05). (B) Islet apoptosis was measured with TUNEL staining after islet isolation. (P<0.05). (C, D, E and F) Fractional beta-cell viability staining using Newport Green, 7-aminoactinomycin D (7-AAD) and tetramethylrhodamine ethyl ester (TMRE) (P<0.05).
