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Methods
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| Participants/experimental animals |
(a) Provide clear details of eligibility criteria in relation to strain, weight range, age range, etc. in Methods section of manuscript; (b) provide description of any run-in testing of suitability of animals for the main experiment; (c) clearly define primary and secondary outcome measures. |
| Sample size |
(a) Provide justification of sample size selection and whether this was determined a priori (based on prespecified primary outcome). |
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Randomization
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| Randomization sequence generation |
(a) Report details of method of generating randomization sequence, including details of stratification if used. |
| Allocation concealment mechanism |
(a) Provide details of whether the persons generating the randomization schedule were blinded to treatment. |
| Blinding |
(a) Provide details of whether persons carrying out randomization, data collection, and data analysis were unaware of treatment group allocation/study hypothesis. |
| Statistical methods |
(a) Indicate any subgroup analysis undertaken and details of how animals came to be included in the subgroup; b. report methods of accounting for non-independence of subjects (e.g. litter mates); c. Indicate whether intent-to-treat analysis was used. |
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Results
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| Attrition |
a. Provide a flow-chart of animals from source population through first allocation to the study, assignment to treatment group, to completion of experiment for each outcome measure; (b) provide description of criteria used for exclusion of animals/data points from analysis and whether these were determined a priori; (c) provide explanations for discrepancies in numbers between experiments/outcome measures (attrition, missing data); (d) provide explanation of process for reviewing erroneous data and whether this was undertaken blinded to treatment group. |
| Baseline data |
Provide details of the animals used, including species, strain, sex, developmental stage, and weight for each experimental group (preferably in tabular form). |
| Numbers analysed |
(a) Report number of animals in each group included in each analysis and whether this was by original assigned groups. |
