Table 1. Summary of the microsatellite libraries prepared in this study and sequencing results showing species name, library preparation method, pooling ratios indicated as portion of a PicoTiterPlate, sequence of the Multiplex Identifier (MID) adaptors and absolute and relative (%) numbers of reads obtained from each library.
Species | Method | Portion | MID | # Reads | Relative # Reads |
Sequencing Run 1 | |||||
Mulloidichthys vanicolensis | shotgun | 1/32 | ACGCGTCTAGT | 33962 | 11.3 |
Mulloidichthys flavolineatus | shotgun | 1/32 | ACGAGTAGACT | 28135 | 9.4 |
Paracirrhites arcatus 1 | enriched | 1/32 | ACACGTAGTAT | 35945 | 8.6 |
Paracirrhites arcatus 2 | enriched | 1/32 | ACACGACGACT | 36551 | 12.2 |
Protoreaster nodosus | enriched | 1/16 | ACGACACGTAT | 85404 | 28.4 |
Acanthaster planci | enriched | 1/16 | ACACTACTCGT | 83835 | 27.9 |
Unmatched | 6731 | 2.2 | |||
Total | 310563 | ||||
Sequencing Run 2 | |||||
Haloptilus longicornis | shotgun | 1/16 | ACGAGTAGACT | 64916 | 24.7 |
Pleuromamma xiphias | shotgun | 1/16 | ACGCGTCTAGT | 75702 | 28.7 |
Octopus cyanea | shotgun | 1/16 | ACGTACTGTGT | 56233 | 21.4 |
Octopus oliveri | shotgun | 1/16 | ACGTACACACT | 59445 | 22.6 |
Unmatched | 7041 | 2.7 | |||
Total | 263337 |
Pink morphotype,
Brown morphotype.