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. 2013 Feb 12;8(2):e55990. doi: 10.1371/journal.pone.0055990

Table 1. Summary of the microsatellite libraries prepared in this study and sequencing results showing species name, library preparation method, pooling ratios indicated as portion of a PicoTiterPlate, sequence of the Multiplex Identifier (MID) adaptors and absolute and relative (%) numbers of reads obtained from each library.

Species Method Portion MID # Reads Relative # Reads
Sequencing Run 1
Mulloidichthys vanicolensis shotgun 1/32 ACGCGTCTAGT 33962 11.3
Mulloidichthys flavolineatus shotgun 1/32 ACGAGTAGACT 28135 9.4
Paracirrhites arcatus 1 enriched 1/32 ACACGTAGTAT 35945 8.6
Paracirrhites arcatus 2 enriched 1/32 ACACGACGACT 36551 12.2
Protoreaster nodosus enriched 1/16 ACGACACGTAT 85404 28.4
Acanthaster planci enriched 1/16 ACACTACTCGT 83835 27.9
Unmatched 6731 2.2
Total 310563
Sequencing Run 2
Haloptilus longicornis shotgun 1/16 ACGAGTAGACT 64916 24.7
Pleuromamma xiphias shotgun 1/16 ACGCGTCTAGT 75702 28.7
Octopus cyanea shotgun 1/16 ACGTACTGTGT 56233 21.4
Octopus oliveri shotgun 1/16 ACGTACACACT 59445 22.6
Unmatched 7041 2.7
Total 263337
1

Pink morphotype,

2

Brown morphotype.