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. 2004 Feb 9;101(7):2094–2099. doi: 10.1073/pnas.0308615100

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Fig. 7. — Intracellular recombination of Ad/HPV16/eGFP in differentiated human airway epithelia. Epithelia were infected with Ad/HPV16/eGFP (A), Ad/HPV16/eGFP plus Ad/LacZ (B), or Ad/HPV16/eGFP plus Ad/Cre (C). Images (×10 magnification) are representative of triplicate infections, with the experiment repeated multiple times. (D) PCR results demonstrating intracellular recombination of Ad/HPV16/eGFP. PCR amplification was performed over the recombination site for HPV. The 100-bp ladder (lane 1), uninfected HAE control (lane 2), HAE infected with Ad/HPV16/eGFP alone (lane 3), HAE infected with Ad/HPV16/eGFP and Ad/Cre (lane 4; amplimer is 35-bp larger because it contains the LoxP sequence as compared with the cloned HPV16 positive control), cloned HPV16 DNA as a positive control (lane 5), and no template control (lane 6).