Figure 2. Expression and purification of SQR, SQRT1 and SQRT2.
SDS-PAGE gel showing the purified C. maquilingensis SQR (WT), along with the water-soluble truncated forms of the enzyme. T1 has the last 21 amino acids removed, including the C-terminal amphiphilic helix, and T2 lacks 45 amino acids and both amphiphilic helices.