Table 1.

Targeting Efficiency of TALENs at 16 Loci in 15 Genes in Various Cell Types

Gene	Target Sequence (Flanked by Underlined TALEN Binding Sites)	Cell Linea	Number of Clones Screened	Number of Mutants	Efficiency of Mutation
AKT2	TCCCTTCCTGCCTCATTTCAGGTGAATACATCAAGACCTGGAGGCCA	HUES 9	192	17	8.9%
AKT2 E17K	TCCCTTCCTGCCTCATTTCAGGTGAATACATCAAGACCTGGAGGCCA	HUES 9	192	3b	1.6%
ANGPTL3	TCAAAACTTGAAAGCCTCCTAGAAGAAAAAATTCTACTTCAACAAAA	HUES 9	424	84	19.8%
APOB	TAAGCGACTGGCTGCCTATCTTATGTTGATGAGGAGTCCTTCACA	HuH-7	126	9	7.1%
ATGL	TTGCTGGCGTTGCCCCCCGCCCGCCCCCACGGCCCAGAGGACAAGGA	HUES 9	192	28	14.6%
C6orf106	TGGAGGGCATGGACGTAGACCTGGACCCGGAGCTGATGCAGAAGTTCA	HUES 9	216	13	6.0%
CIITA	TAACAGCGATGCTGACCCCCTGTGCCTCTACCACTTCTATGACCAGA	BJRiPS	292	37	12.7%
CELSR2	TGCTGGCTCGGCTGCCCTGAGGTTGCTCAATCAAGCACAGGTTTCAA	HUES 1	506	18	3.5%
CFTR	TGGAATCACACTGAGTGGAGGTCAACGAGCAAGAATTTCTTTAGCA	CF-RiPS	140	3	2.1%
GLUT4	TGGTCCTTGCTGTGTTCTCTGCGGTGCTTGGCTCCCTGCAGTTTGGGTA	HUES9	155	52	33.5%
LINC00116	TCAGAGAGGACACTGCAGTTGTCCGTGCTAGTAGCCTTCGCTTCTGGA	HUES 9	88	26	29.5%
NLRC5	TGGAGCTGTCTTGTGAGGCTGCTCACCAAAGACCCAGAATGGCTGAA	BJ-RiPS	83	6	7.2%
PLIN1	TGACAACGTGGTGGACACAGTGGTGCATTACGTGCCGGTGAGTACCA	HUES 9	293	70	23.8%
PLIN1	TGACAACGTGGTGGACACAGTGGTGCATTACGTGCCGGTGAGTACCA	BJ-RiPS	439	29	6.6%
SORT1 (exon 2)	TGATGATCTCAGAGGCTCAGTATCCTTGTCCTGGGTTGGAGATAGCA	HUES 1	576	128	22.2%
SORT1 (exon 3)	TGGTAATTATGACTTTTGGACAGTCCAAGCTATATCGAAGGTGAGATCA	HUES 9	192	21	10.9%
TRIB1	TCTTCAAGCAGATTGTCTCCGCCGTCGCCCACTGCCACCAGTCAGCCA	HUES 9	169	41	24.2%
TTN	TTGAGGCTCCTTAATGATGACAGAAGAGAAGGCTTCTCTGGGTTCA	BJ-RiPS	250	17	6.8%

^aHUES 1 and HUES 9 are human embryonic stem cell lines (Cowan et al., 2004); BJ-RiPS and CF-RiPS are induced pluripotent stem cell lines (Warren et al., 2010); HuH-7 cells are cultured human hepatocellular carcinoma cells

^bSuccessfully inserted E17K knock-in mutation using single-stranded DNA oligonucleotide