Figure 4. Hsp90Ec mutants are defective in client binding.
(A) The ATPase activity of Hsp90Ec wild type or mutants with or without L2 was measured. Data from 3 replicates are presented as mean ± SEM.
(B) Binding of Hsp90Ec wild type or mutants to IAEDANS-labeled Δ131Δ was measured by fluorescence anisotropy. Binding curves are the average of two independent measurements.
See also Figure S4.