Skip to main content
. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Neurochem Res. 2012 Oct 18;38(6):1100–1112. doi: 10.1007/s11064-012-0906-y

Figure 4. PAX6 is an early marker of pineal development and is only detectable at low levels in the adult.

Figure 4

A) In situ hybridization for detection of Pax6 mRNA in a sagittal section of the rat brain at E16. Note the strong signal in the epithelium around the pineal recess (arrow). Di, diencephalon; Mes, mesencephalon; Rhomb, rhombencephalon; Tel, telencephalon. Scale bar, 1 mm. B) Immunohistochemical detection of PAX6 protein in the developing rat pineal gland at E17. Scale bar, 50 μm. The fetal animal was not perfused; therefore, the very dark cells are erythrocytes with a high level of endogenous peroxidase. C) Immunohistochemical detection of PAX6 protein in the pineal gland of a perfusion fixed adult rat. Scale bar, 50 μm. For methodological details, see [76].