Table 1.
Antibodies Used and Their Immunogens, Suppliers, and Dilutions.
| antigen | immunogen | manufacturer, species, type, catalog number | Dilution used |
|---|---|---|---|
| Choline acetyltransferase | human placental enzyme | Millipore, Inc. (Billerica, MA) goat polyclonal #AB144P | 1:100 |
| RIBEYE | CtBP2 C-term. a.a. 361–445 |
BD Biosciences (San Jose, CA) mouse monoclonal, #612044 | 1:500 |
| Calbindin D-28 (CB) | recombinant rat CB | Swant (Switzerland) rabbit polyclonal, #CB38 | 1:5,000 |
| Tyrosine hydroxylase (TOH) | rat TOH a.a. 40–152 |
Sigma, Inc. (St. Louis, MO) mouse monoclonal #T2928 | 1:10,000 |
| GABA | formaldehyde conjugate - porcine thyroglobulin | Immunosolution Pty Ltd (Jesmond, NSW, AU) rabbit polyclonal #IG1004 | 1:1,000 |