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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Immunol Cell Biol. 2012 Dec 18;91(2):139–148. doi: 10.1038/icb.2012.66

Figure 1. Enhanced protection is observed using anti-iFt mAb + iFt ICs.

Figure 1

Prior to immunization, 1×109 iFt organisms were incubated at 4°C overnight with 0, 1 or 5 μg/ml of anti-iFt mAb in PBS. Following the incubation, iFt and mAb + iFt ICs were washed (to remove free mAb) and analyzed by SDS PAGE and Western Blot to verify mAb-iFt IC formation (A). 2×107 iFt organisms alone or complexed with anti-iFt mAb were administered i.n. to C57BL/6 mice. The mice were boosted on day 21 and challenged on day 35 with either 20,000 CFU of Ft LVS (B) or boosted on days 14 and 28 and challenged on day 42 with 21 CFU of Ft SchuS4 (C). Survival was monitored for 21 days. The p value for iFt versus mAb + iFt ICs in B (Ft LVS challenge) was < 0.05. The p value for iFt versus mAb + iFt ICs in C (Ft SchuS4 challenge, 5 μg) was < 0.02.