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Decomplexation (or pDNA release) of PRLx polyplexes (N/P 5). After the polyplexes with pDNA (25 μg/mL) were exposed to DTT (20 mM) and heparin (0–100 μg/mL) in 150 mM NaCl aqueous solution at 37°C for 1 hr, the polyplex solution was loaded into 0.8% agarose gel and then was electrophoresed at 80 V for 90 min.
