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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: J Invest Dermatol. 2012 Oct 25;133(3):759–767. doi: 10.1038/jid.2012.355

Figure 1. Downregulation of melanoma-associated antigen-encoding (MAGE)-C2 induces apoptosis in A375 melanoma cells.

Figure 1

A375 cells were transduced with lentiviruses expressing tetracycline-responsive scrambled or MAGE-C2 short hairpin RNA (shRNA) and red fluorescent protein (RFP). Transduction efficiency was assessed by RFP expression and stained for MAGE-C2 (green). (a) A375 cells expressing MAGE-C2 shRNA before selection with puromycin were treated with 0.5 μgml−1 doxycycline for 72 hours and showed decreased MAGE-C2 expression (green) in RFP-positive cells. Bar = 50 μm. (b) A histogram of MAGE-C2 intensity in RFP-expressing versus non-expressing cells using the NIS Elements software, *P<0.05. (c) Single-cell clones were selected on the basis of RFP and MAGE-C2 expression. MAGE-C2 expression in a representative clone is shown by immunoblotting after 72 hours of 0.5 μgml−1 doxycycline treatment. (d) Downregulation of MAGE-C2 induces apoptosis in A375 cells as analyzed by TUNEL assay, *P<0.02.