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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: J Invest Dermatol. 2012 Oct 25;133(3):759–767. doi: 10.1038/jid.2012.355

Figure 3. Melanoma-associated antigen-encoding (MAGE)-C2 knockdown reduces growth of MRA-13 melanoma xenografts in athymic nude mice.

Figure 3

Four-week-old athymic nude mice, seven mice per group, were injected with MRA-13 cells expressing scrambled or MAGE-C2 short hairpin RNA (shRNA) and were given either water or 2mgml−1 doxycycline solution. The xenograft experiment was terminated 6 weeks later or when tumors reached a volume of 1,400 mm3. (a) Tumor growth decreased by 40% in MAGE-C2 shRNA mice treated with doxycycline (MAGE-C2 downregulation) compared with no doxycycline treatment. (b) Linear trend lines compare growth of xenografts expressing MAGE-C2 and scrambled shRNA with their counterparts without doxycycline treatment. MAGE-C2 shRNA tumors show a downward trend compared with scrambled shRNA tumors. (c) Immunoperoxidase staining shows that MAGE-C2 expression in xenograft tumors is decreased by doxycycline-dependent induction of MAGE-C2 shRNA. Bar = 50 μm. (d) Downregulation of MAGE-C2 is observed by immunoblotting in MAGE-C2 shRNA tumors after doxycycline treatment. (e) Apoptosis was detected in xenograft tumor sections by TUNEL assay. The histograms show increase in apoptosis in tumors with MAGE-C2 downregulation, *P<0.05.