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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: J Invest Dermatol. 2012 Oct 25;133(3):759–767. doi: 10.1038/jid.2012.355

Figure 4. Melanoma-associated antigen-encoding (MAGE)-C2 induces phosphorylation of KAP1-Ser824.

Figure 4

A375 cells expressing scrambled or MAGE-C2 short hairpin RNA (shRNA) were treated with doxycyclin and DNA-damaging agent, doxorubicin. HEK293T cells were transfected to ectopically express MAGE-C2 and treated with doxorubicin. (a) Immunoblotting shows that doxorubicin-induced phosphorylation of KAP1-Ser824 is suppressed by MAGE-C2 knockdown in A375 cells expressing MAGE-C2 shRNA and treated with doxycycline. (b) In HEK293T cells, overexpression of MAGE-C2 induces KAP1-Ser824 phosphorylation and also enhances doxorubicin-induced phosphor-KAP1-Ser824.