Table 1.
Library No. (sequence) |
Theoretical diversity |
Fast Cyclization Sequences | Slow Cyclization Sequences | |||||||
---|---|---|---|---|---|---|---|---|---|---|
No of beads screened |
No. of colorless beads at 2 or 5 min (%) |
No. of beads sequenced (No. of full sequences) |
No of beads with dimer formation |
No. of beads screened |
No. of colored beads at 90 min (%) |
No. of full sequences |
No. of beads with dimer formation |
|||
I | X3E | 8.0 × 103 | 30,000 | 75 | 20 (11) | 6 (54%) | 30,000 | 25 (0.083) | 15 | 2 (13%) |
II | X4E | 1.6 × 105 | 60,000 | 70 | 20 (11) | 8 (73%) | 60,000 | 13 (0.02) | 7 | 1 (14%) |
III | X5E | 3.2 × 106 | 210,000 | 65 | 101 (46) | 2 (4.3%) | 210,000 | 75 (0.036) | 50 | 1 (2.0%) |
IV | X6E | 6.4 × 107 | 210,000 | 65 | 89 (61) | 0 (0%) | 210,000 | 51 (0.024%) | 34 | 0 (0%) |
V | X7E | 1.3 × 109 | 420,000 | 60 | 126 (109) | 1 (0.9%) | 420,000 | 166 (0.04%) | 157 | 0 (0%) |