Table 3.
Kinetic parameters of l-histidinol phosphatases from cog 1387.
| Locus tag | Organism | kcat (s−1) | Km (mM) |
kcat/Km (M−1s−1) |
Metal content per enzyme monomer |
|---|---|---|---|---|---|
| MCCL_0344 | Macrococcus caseolyticus JCSC5402 | 30 ± 2 | 1.5 ± 0.3 | (2.0 ± 0.2) × 104 | Mn (0.9); Fe (0.6); Zn (0.3) |
| BBR47_00270 | Brevibacillus brevis NBRC 100599 | 7.2 ± 0.4 | 2.6 ± 0.3 | (2.8 ± 0.2) × 103 | Zn (1.6); Mn (0.3) |
| BCE_1533 | Bacillus cereus ATCC10987 | 8.1 ± 0.1 | 1.5 ± 0.1 | (5.6 ± 0.2) × 103 | Mn (1.4); Zn (0.4) |
| BcerKBAB4_1335a | Bacillus weihenstephanensis KBAB4 | 1.2 ± 0.1 | 4.9 ± 0.3 | (2.5 ± 0.2) × 102 | Zn (1.5); Fe (1.2) |
| BcerKBAB4_1335 | 34 ± 2 | 1.3 ± 0.2 | (2.6 ± 0.2) × 104 | Mn (1.0); Zn (0.6) | |
| BSU29620a | Bacillus subtilis subsp. subtilis str. 168 | 0.37 ± 0.01 | 0.10 ± 0.01 | (3.7 ± 0.6) × 103 | Fe (2.6); Zn (0.4) |
| BH3206 | Bacillus halodurans C-125 | 28 ± 1 | 0.64 ± 0.08 | (4.4 ± 0.5) × 104 | Mn (1.1); Zn (0.3) |
| GK2799 | Geobacillus kaustophilus HTA426 | 7.6 ± 1.4 | 8 ± 3 | (9.0 ± 1.1) × 102 | Mn (1.0); Zn (0.4); Fe (0.3) |
| SMU_1486c | Streptococcus mutans UA159 | 0.31 ± 0.01 | 0.58 ± 0.07 | (5.3 ± 0.8) × 102 | Zn (0.4); Fe (0.3) |
a
Enzymes were over-expressed without the addition of 2,2’-bipyridyl in the growth medium.