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. Author manuscript; available in PMC: 2014 Mar 15.
Published in final edited form as: Toxicon. 2013 Jan 10;64:43–54. doi: 10.1016/j.toxicon.2012.12.025

Fig 3.

Fig 3

SDS-PAGE of the partially purified r-Cam-dis (a) and cleaved empty pET-43.1a vector (b) under non-reducing condition. Samples were run on 4–12% Bis-Tris Gel using an Xcell SureLock Mini-Cell at 200 V for 30 min. The gel was stained with RapidStain. a) Lane 1: SeeBlue Plus2 Markers; lane 2: the partially purified r-Cam-dis (4 μg). b) Lane 1: SeeBlue Plus2 Markers; lane 2: cleaved empty vector pET-43.1a (3 μg); lane 3: purified cleaved Nus tag (1 μg). The sequence for each band and its identification are on the right. An asterisk (*) represents the N-terminal amino acid sequence of purified r-Cam-dis containing the nine amino acids from the vector (italicized) before the disintegrin sequence (bold).