Fig. 4.

Inhibition of platelet adhesion to fibrinogen by r-Cam-dis. Platelet (10 × 10⁶ cells) were preincubated with various concentrations of r-Cam-dis at 37°C for 1 h prior to adding to fibrinogen-coated wells. p-nitrophenyl phosphate containing 0.1% Triton X-100 was used as the substrate solution. The reaction product p-nitrophenol was measured at 405 nm. The IC₅₀ was 0.01 μg/mL (an estimated IC₅₀ value of 1 nM). The cleaved Nus-tag (~14 kDa; ▲) had no effect on platelet adhesion. The vertical bars represent the standard deviation. n = 3.