Fig. 5.

Inhibition of platelet aggregation induced by collagen (a) and ADP (b) with r-Cam-dis and r-mojastin-1. The inhibition assay was performed with human whole blood as described in the Materials and Methods. Platelet aggregation was initiated by a 5 μg/mL of collagen or a final concentration of 10 μM ADP. The maximal aggregation in the absence of recombinant disintegrin was given as 100% aggregation. The IC₅₀s values for inhibiting collagen-induced platelet aggregation were 0.3 μg/mL (an estimated IC₅₀ of 18 nM) for r-Cam-dis and 3 μg/mL (an IC₅₀ of 341 nM) for r-mojastin-1. The IC₅₀s values for inhibiting ADP-induced platelet aggregation were 0.1 μg/mL (an estimated IC₅₀ of 6 nM) for r-Cam-dis and 0.2 μg/mL (an IC₅₀ of 24 nM) for r-mojastin-1. The cleaved Nus-tag (~14 kDa; ▲) did not inhibit collagen- and ADP-induced platelet aggregation. The vertical bars represent the standard deviation. n = 3.