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How Features of Light Can Affect Measurements of Fluorescent Lifetime and Spectroscopya
| Features of incident light | FL | Spectroscopy |
|---|---|---|
| Fluctuations in incident light signal amplitude | No | Yes |
| Duration of incident light exposure | No | Yes |
| Fluorophore concentration | No | Yes |
| Fluorescence intensity | No | Yes |
| Emission spectrum | No | Yes |
| Light scattering by tissue | No | Yes |
No, effect is absent; yes, effect is present.
