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Fluorescence lifetime imaging of C2C12 cells expressing AcGFP1-GBPcys-mCherry and AcGFP1-GBPcys, respectively. C2C12 cells were transfected transiently with either pcDNA3.1-AcGFP1-GBPcys-mCherry (A) or pcDNA3.1-AcGFP1-GBPcys (B). FD-FLIM was performed at 48 h post- transfection. Lifetime Lookup Table: 0.0–4.0 ns
