Fig. 3.

ICV infusion of alcohol impairs systemic leucine response. (A), Muscle protein synthesis in rats infused ICV with aCSF (control) or alcohol for 24 h prior to intragastric administration of saline or leucine. (B) Representative western blots for phosphorylated 4E-BP1 and S6K1 in the gastrocnemius removed from rats in the four experimental groups. Arrow to the right of blot indicates the most highly phosphorylated and active γ-isoform of 4E-BP1. Samples from saline- and leucine-treated were run on the same gel, but selected lanes were deleted for clarity and the deletion is indicated by the white line between blots. Values are means ± SEM; n = 6–8 rats per group. Values with a different letters are statistically different (P < 0.05).