Table 1. Thermodynamic Parameters Calculated from UV Melting in NaCl, KCl, and LiCl at pH 7.0 Buffered with 10 mM MOPS and 0.1 mM EDTA.
| hairpin | salt | TM (°C)a | ΔH° (kcal/mol) | ΔS° (eu) | ΔG°37(kcal/mol)a,b | ΔG°37,HL(kcal/mol)c |
|---|---|---|---|---|---|---|
| r(5′GGACCCCCGUCC) | 1 M NaCl | 61.9 | –31.8 | –94.9 | –2.37 | 6.48 |
| 1 M KCl | 62.7 | –30.6 | –91.1 | –2.35 | 6.50 | |
| 1 M LiCl | 64.8 | –31.5 | –93.2 | –2.59 | 6.26 | |
| r(5′GGACUUUUGUCC) | 1 M NaCl | 67.9 | –42.7 | –125.2 | –3.87 | 5.18 |
| 1 M KCl | 66.1 | –40.3 | –118.8 | –3.45 | 5.60 | |
| 1 M LiCl | 68.6 | –41.8 | –122.3 | –3.87 | 5.18 | |
| r(5′GGACCCCCGUCC) | 5 mM NaCl | 57.3 | –30.3 | –91.7 | –1.86 | – |
| 10 mM KCl | 57.1 | –32.4 | –98.1 | –1.97 | – | |
| 10 mM LiCl | 58.5 | –32.4 | –97.7 | –2.10 | – | |
| r(5′GGACUUUUGUCC) | 5 mM NaCl | 58.8 | –38.7 | –116.6 | –2.54 | – |
| 10 mM KCl | 61.6 | –39.3 | –117.4 | –2.89 | – | |
| 10 mM LiCl | 60.2 | –38.5 | –115.5 | –2.68 | – |
a
Tm and ΔG°37 were calculated after averaging the ΔH° and ΔS° results.
b
Folding free energy for the stem–loop structure.