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Elderly PBMC secrete relatively low levels of IFN-γ in conjunction with secreting high basal levels of IL-13 ex vivo. PBMC cultures (n = 10 young and n = 10 elderly) were incubated in medium alone or with RSV F protein, and the resulting cytokines secreted into the supernatant were measured using a multiplex Th1-Th2 cytokine bead array panel (Luminex). (A) IFN-γ. (Β) IL-13.
