Fig 4.

Immunization with rApfA protein protects mice against lethal challenges with strain 4074 (serovar 1) and strain WF83 (serovar 7). (A) A high level of specific anti-rApfA IgG antibody was induced in mice by immunization with rApfA. (B and C) Survival of mice immunized with rApfA, bacterin (positive control), and adjuvant (negative control) following i.p. infection with (B) strain 4074 (serovar 1) and (C) strain WF83 (serovar 7). The data shown are representative of the results of one of three independent experiments; the survival data were analyzed using the log rank (Mantel-Cox) test. The asterisks show significant differences (**, P < 0.01).