Table 1.
Characteristics of bacterial strains and primers used in this study
| Strain, plasmid, or primer | Relevant characteristics or sequence | Source or reference |
|---|---|---|
| Strains | ||
| 4074 | A. pleuropneumoniae reference strain of serovar 1, apfA positive | P. Blackall |
| WF83 | A. pleuropneumoniae reference strain of serovar 7, apfA positive | P. Blackall |
| 4074ΔapfA | A. pleuropneumoniae 4074 apfA mutation strain | This work |
| C4074ΔapfA | A. pleuropneumoniae apfA complementary strain | This work |
| Plasmids | ||
| pET-30a(+) | Expression vector, kanamycin resistant | Novagen |
| pJN105-sodC | Shuttle vector pJN105 with a sodC promoter, gentamicin resistant | 17 |
| pEMOC2 | Suicide vector, chloramphenicol resistant | 18 |
| Primers | ||
| apfA01 | 5′-CGTACGGTCGACGAATTGATGATCGTGATTGC-3′ | This work |
| apfA02 | 5′-CGCTAGGCGGCCGCTAAGTCCTCCCCTTTACATT-3′ | This work |
| apfA03 | 5′- CGTACGGAATTCATGCAAAAACTAAGTCTTATTC-3′ | This work |
| apfA04 | 5′- CGCTAGTCTAGATTAATTTGATGCGCAGAAATT-3′ | This work |
| apfA05 | 5′-CGTACGGAATTCTTTACTTTAATTGAATTGATGAT-3′ | This work |
| apfA06 | 5′-CGCTAGGTCGACTTAATTTGATGCGCAGAAATT-3′ | This work |
| apfA-S | 5′-CGAGATCTGCATATATAACACCG-3′ | This work |
| apfA-A | 5′-CAGAAATTTGCCGGAAATAAAC-3′ | This work |
| apfB-S | 5′-TTCGGGAAGACCAACAAACA-3′ | This work |
| apfB-A | 5′-TAAACAGGCCGTCCAACACT-3′ | This work |
| apfC-S | 5′-AACAAAGCGGGCGATTAGG-3′ | This work |
| apfC-A | 5′-GGCACGATAAAAAGGAGCATG-3′ | This work |
| ackA-S | 5′-GCGGAAGATAACTACGAGGACG-3′ | This work |
| ackA-A | 5′-AATGTTGAGTCGTCCGCAGTG-3′ | This work |