Fig 1.

Identification of strong MAV2054 and MAV5183 seroreactivity in MAC-PD patients. An ammonium sulfate precipitate of M. avium CFP was fractionated by hydrophobic interaction chromatography using phenyl Sepharose. Each of the primary fractions was further fractionated by hydroxyapaptite chromatography. The third fractionation was conducted by DEAE ion-exchange chromatography. IgG titers in all fractions of sera from MAC-PD, pulmonary TB, and HC subjects were determined by ELISA. Two fractions, F15 (A) and F30 (B), were selected, subjected to SDS-PAGE, and stained with Coomassie brilliant blue.