Fig 2.

Kch1 and Kch2 are essential for maintaining cell viability. (A) The strains listed in Fig. 1C were exposed to 25 μM α-factor in SC-100 media with or without additional 100 mM CaCl₂ and stained with propidium iodide. Live and dead cells were counted by flow cytometry. (B) Strains kch1 kch2 and cch1 kch1 kch2 (CS03 and CS07) were transformed with a control plasmid or plasmids that overexpress the KCH2-HA₃ or KCH1-HA₃ genes (pSM10, pCS01, and pCS02), exposed to 50 μM α-factor, and then analyzed for pheromone-induced cell death as in panel A. The averages of three biological replicates (± the SD) are shown.