Table 2.
Plasmids used in this study
| Plasmid | Description | Source or reference |
|---|---|---|
| pJB207 | 2μ URA3 FUS1::lacZ | 29 |
| pEVP11/AEQ89 | 2μ LEU2 PADH-aequorin | 30 |
| pSM10 | CEN LEU2 PMET25-HA3 | 31 |
| pCS01 | CEN LEU2 PMET25-KCH2-HA3 | This study |
| pCS02 | CEN LEU2 PMET25-KCH1-HA3 | This study |
| pCS42 | CEN URA3 PMET25-HA3 | This study |
| pCS43 | CEN URA3 PMET25-KCH2-HA3 | This study |
| pCS44 | CEN URA3 PMET25-KCH1-HA3 | This study |
| pEG311 | URA3 PTDH3-GFP | 51 |
| pCS47 | URA3 PTDH3-GFP-KCH2-HA3 | This study |
| pCS48 | URA3 PTDH3-GFP-KCH1-HA3 | This study |
| pEGFP-C3 | PCMB-EGFP | Clontech |
| pCS49 | PCMB-EGFP-KCH2 | This study |
| pCS50 | PCMB-EGFP-KCH1 | This study |