Fig 5.
The set of fungicidal anticryptococcal drugs contains a number of calmodulin antagonists. (A) Thermal shift assay for CnCam1 in the presence of DMSO or the well-characterized calmodulin antagonist triflupromazine (TFP; 100 μM). The x axis shows the temperature, and the y axis shows the negative derivative of the fluorescence value. The red arrow indicates the Tm for CnCam1 in the presence of DMSO, and the green arrow indicates that in the presence of TFP. (B) Changes in Tm induced by the indicated drugs relative to that with DMSO (1%). The bars indicate means for three independent experiments performed in triplicate; error bars indicate standard deviations.