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UME6 expression level is correlated with increased biofilm formation. Suspensions of 1 × 106 cells/ml of the indicated strains were allowed to form biofilms on 96-well polystyrene plates in Lee's pH 6.8 medium at 30°C either in the absence of Dox or in the presence of the indicated Dox concentrations. Biofilm formation was assessed by using a standard colorimetric XTT reduction assay (18, 48). Error bars represent standard deviations (n = 4).
