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UME6 expression drives C. albicans tissue invasion in a reconstituted three-dimensional model of oropharyngeal candidiasis. A three-dimensional organotypic model of the human oral mucosa was infected with 1 × 105 cells of the indicated C. albicans strains in the presence or absence of 20 μg/ml Dox. Following a 24-h infection period, cultures were fixed in formaldehyde, embedded in paraffin, stained with H&E, and visualized by light microscopy. Bar = 100 μm.
