Fig 4.

Hgc1 and Sun41 are important for the ability of UME6 expression to cause enhanced biofilm formation. Suspensions of 1 × 10⁶ cells/ml of the indicated strains were allowed to form biofilms for 24 h on 96-well polystyrene plates in Lee's medium, pH 6.8, (A) or in minimal medium (B) at 30°C in the presence or absence of 20 μg/ml Dox. Biofilm formation was assessed by using a standard colorimetric XTT reduction assay (18, 48). Error bars represent standard deviations (n = 8).