Fig 6.
IdeSsuis cleaves IgM from pigs but not IgM from humans, mice, and warthogs. (A) Anti-IdeSsuis Western blot analysis of 24-fold-concentrated supernatants of S. suis strains 10 (wt), 10ΔideSsuis (Δ), and 10ΔideSsuis(pGA14ideSsuis) (c) as well as the 6-fold-concentrated supernatants of L. lactis pOri23 (p) and L. lactis(pOriideSsuis) (h). (B to F) Anti-IgM Western blot analysis of diluted plasma or serum from pig (B and C), human (D), mouse (E), and warthog (F) incubated with the concentrated supernatants investigated as shown in panel A or with PBS. Detection of IgM was conducted with polyclonal antibodies (PAB) against porcine (B), human (D), or mouse (E) IgM or with a monoclonal antibody (MAB) against porcine IgM (C and F). The positions of the IgM multimers are marked with arrows on the left side of the blots, and the cleavage products of porcine IgM are indicated by asterisks on their left side. The final dilution of porcine plasma or serum was 1:100 in all samples. The marker bands are shown on the left (sizes in kDa).