Fig 8.
The ideSsuis gene is transcribed ex vivo in porcine blood (A), and IdeSsuis promotes increased survival in blood of a piglet vaccinated once with a bacterin (B). (A) RT-PCR detection of ideSsuis mRNA of S. suis strain 10 in vitro grown to early exponential (exp.) or stationary (stat.) phase or incubated ex vivo with porcine blood for the indicated times. The marker bands are shown on the left side (sizes in bp). RT, reverse transcriptase. (B) Survival of S. suis strain 10 (wt), strain 10ΔideSsuis (Δ), and strain 10ΔideSsuis(pGA14ideSsuis) (c) in porcine blood, which was drawn from a piglet between 15 and 18 days after immunization with a bacterin (single application).