Fig 2.

Impact of patA1 and patA2 mutations on S-layer protein and S-layer-associated protein trafficking in B. anthracis. (A) B. anthracis vegetative cultures were fractionated into medium (M), S-layer (S), and cell (C) fractions, and proteins in each sample were analyzed by Coomassie-stained SDS-PAGE gels. The arrowhead denotes the migratory position of the S-layer proteins Sap and EA1, and the numbers indicate the position of molecular mass markers (in kDa). (B) Samples generated in the experiment shown in panel A were examined by immunoblotting with antibodies raised against protective antigen (PA), a secreted protein and component of anthrax toxins (42), and PrsA1, a peptidylprolyl isomerase and membrane lipoprotein (43), as well as S-layer proteins (Sap and EA1) and S-layer-associated proteins (BslA and BslO). α, anti.