Fig 5.
SCWP isolated from wild-type and patA1 patA2 mutant B. anthracis strains. (A) The SCWP was released via hydrofluoric acid (HF) treatment from murein sacculi that had been isolated from either wild-type or patA1 patA2 mutant vegetative forms. Ethanol-precipitated SCWP was subjected to rpHPLC, and the eluate's absorbance at 206 nm (A206) was recorded in milli-arbitrary units (mAu). (B) Collected rpHPLC fractions from panel A (A1), were tested for competitive inhibition of EA1SLH-mCherry binding to the SCWP of wild-type bacilli. SCWPs from wild-type but not SCWPs from patA1 patA2 mutant bacilli displayed competitive inhibitor activities toward the binding of bacilli to EA1SLH-mCherry.