Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Descriptiona | Reference or source |
|---|---|---|
| Strains | ||
| B. anthracis | ||
| Sterne 34F2 | (pXO1+ pXO2−) wild type | Laboratory collection |
| JDB2519 | patA1::tetL (Tetr) | 24 |
| JDB2578 | patA2::aad9 (Specr) | 24 |
| JDB1972 | patA1::aphA3::patA2 (Kanr) | 24 |
| JDB2573 | patA1::aphA3::patA2 pML360 | 24 |
| JDB2609 | patA1::aphA3::patA2 pML301 | 24 |
| JML145 | patA1::aphA3::patA2 pAD123 | This work |
| B. subtilis | ||
| PY79 | Wild type | Laboratory collection |
| E. coli | ||
| JWK440 | BL21(DE3), pJK88 | This work |
| Plasmids | ||
| pML301 | pAD123 derivative containing patA2-bas0846 on a BamHI/KpnI fragment; Cmr Ampr | 24 |
| pML360 | pAD123 derivative containing patA1-bas0843 on a BamHI/KpnI fragment; Cmr Ampr | 24 |
| pAD123 | pTA1060 ori, cat Cmr bla Ampr | 35 |
| pJK88 | pET16b plasmid with the first 180 amino acids of eag as a translational fusion with mCherry | This work |
a
Tetr, tetracycline resistance, Specr, spectinomycin resistance; Kanr, kanamycin resistance; Cmr, chloramphenicol resistance; Ampr, ampicillin resistance.