Table 1.
Plasmids and strains used in this study
| Plasmid or strain | Relevant characteristic | Source or reference |
|---|---|---|
| Plasmids | ||
| pAPNC213 | bla aprE5′ spc lacI PspacaprE3′ | 20 |
| pAPNC213cat | bla aprE5′ cat lacI PspacaprE3′ | H. Strahl |
| pDG9 | bla amyE3′ spc Pxyl-divIVA-gfp amyE5′ | 18 |
| pSG1154 | bla amyE3′ spc Pxyl-′gfp amyE5′ | 21 |
| pSH2 | bla aprE5′ spc lacI Pspac-divIVABsaprE3′ | 18 |
| pSH3 | bla amyE3′ spc Pxyl-divIVABs-gfpA206K amyE5′ | 18 |
| pKT25-racA | kan Plac-cya(T25)-racA | 1 |
| pUT18-divIVA | bla Plac-cya(T18)-divIVA | 1 |
| pUT18C-divIVA | bla Plac-cya(T18)-divIVA | 11 |
| pUT18C-minJ | bla Plac-cya(T18)-minJ | 11 |
| pUT18C-racA | bla Plac-cya(T18)-racA | 1 |
| p25-N-divIVA | kan Plac-divIVA-cya(T25) | 1 |
| p25-N-minJ | kan Plac-minJ-cya(T25) | 11 |
| pINC3 | bla amyE3′ spc Pxyl-divIVAR131A-gfp amyE5′ | This work |
| pINC12 | bla aprE5′ spc lacI Pspac-divIVAR131A aprE3′ | This work |
| pKK13 | bla amyE3′ spc Pxyl-divIVABs-57-LmamyE5′ | This work |
| pSBLH001 | bla Plac-divIVA1-459(Δ11)-cya(T18)a | This work |
| pSBLH004 | kan Plac-divIVA1-459(Δ11)-cya(T25) | This work |
| pSBLH005 | bla Plac-divIVA1-432(Δ20)-cya(T18) | This work |
| pSBLH008 | kan Plac-divIVA1-432(Δ20)-cya(T25) | This work |
| pSBLH036 | bla Plac-divIVA1-429(Δ21)-cya(T18) | This work |
| pSBLH037 | bla Plac-divIVA1-414(Δ26)-cya(T18) | This work |
| pSBLH038 | bla Plac-divIVA1-390(Δ34)-cya(T18) | This work |
| pSBLH039 | kan Plac-divIVA1-429(Δ21)-cya(T25) | This work |
| pSBLH040 | kan Plac-divIVA1-414(Δ26)-cya(T25) | This work |
| pSBLH041 | kan Plac-divIVA1-390(Δ34)-cya(T25) | This work |
| pSH19 | bla amyE3′ spc Pxyl-divIVABsamyE5′ | This work |
| pSH209 | bla amyE3′ spc Pxyl-divIVALmamyE5′ | This work |
| pSH210 | bla amyE3′ spc Pxyl-divIVALm-gfp amyE5′ | This work |
| pSH260 | bla amyE3′ spc Pxyl-divIVALm-71-BsamyE5′ | This work |
| pSH261 | bla amyE3′ spc Pxyl-divIVALm-144-BsamyE5′ | This work |
| pSH267 | bla amyE3′ spc Pxyl-divIVALm-130-BsamyE5′ | This work |
| pSH272 | bla amyE3′ spc Pxyl-divIVALm-83-BsamyE5′ | This work |
| pSH278 | bla amyE3′ spc Pxyl-divIVALm-104-BsamyE5′ | This work |
| pSH290 | bla amyE3′ spc Pxyl-divIVALm-71-Bs-gfpA206K amyE5′ | This work |
| pSH291 | bla amyE3′ spc Pxyl-divIVALm-144-Bs-gfpA206K amyE5′ | This work |
| pSH292 | bla amyE3′ spc Pxyl-divIVALm-130-Bu-gfpA206K amyE5′ | This work |
| pSH293 | bla amyE3′ spc Pxyl-divIVALm-83-Bs-gfpA206K amyE5′ | This work |
| pSH294 | bla amyE3′ spc Pxyl-divIVALm-104-Bs-gfpA206K amyE5′ | This work |
| pSH316 | bla aprE5′ cat lacI Pspac-minJ-gfp aprE3′ | This work |
| pSH317 | bla aprE5′ cat Pspac-minJ-gfp aprE3′ | This work |
| pSH320 | bla aprE5′ cat PdivIVA1-minJ-gfp aprE3′ | This work |
| pSH326 | bla amyE3′ spc Pxyl-divIVABs-57-lm-gfp amyE5′ | This work |
| pSH328 | bla aprE5′ cat PdivIVA3-minJ-gfp aprE3′ | This work |
| pSH330 | bla aprE5′ spc lacI Pspac-divIVABsR102K aprE3′ | This work |
| pSH331 | bla aprE5′ spc lacI Pspac-divIVABsR102E aprE3′ | This work |
| pSH334 | bla aprE5′ spc lacI Pspac-divIVABsΔC34 aprE3′ | This work |
| pSH335 | bla aprE5′ cat lacI Pspac-divIVABsaprE3′ | This work |
| pSH336 | bla aprE5′ cat lacI Pspac-divIVABsR102K aprE3′ | This work |
| pSH337 | bla aprE5′ cat lacI Pspac-divIVABsR102E aprE3′ | This work |
| pSH340 | bla aprE5′ cat lacI Pspac-divIVABsΔC34 aprE3′ | This work |
| pSH354 | bla amyE3′ spc Pxyl-divIVALm-gfpA206K amyE5′ | This work |
| pSH355 | bla amyE3′ spc Pxyl-divIVABs-57-Lm-gfpA206K amyE5′ | This work |
| B. subtilis strains | ||
| 168 | trpC2 | |
| 168(pSG4916) | 168 racA::pSG4916(Pxyl-gfp-racA′ cat) | 8 |
| 4041 | 168 divIVA::tet | 18 |
| BSN5 | 168 aprE::Pspac-divIVA spc lacI divIVA::tet | 18 |
| SB002 | 168 amyE::Pxyl-minJ-gfp spc minJ::tet | 22 |
| BSN51 | 168 amyE::Pxyl-divIVABsspc divIVA::tet | This work |
| BSN238 | 168 amyE::Pxyl-divIVALmspc divIVA::tet | This work |
| BSN274 | 168 amyE::Pxyl-divIVALm-144-Bsspc divIVA::tet | This work |
| BSN278 | 168 amyE::Pxyl-divIVALm-130-Bsspc divIVA::tet | This work |
| BSN287 | 168 amyE::Pxyl-divIVALm-83-Bsspc divIVA::tet | This work |
| BSN288 | 168 amyE::Pxyl-divIVALm-104-Bsspc divIVA::tet | This work |
| BSN294 | 168 amyE::Pxyl-divIVALm-71-Bs-gfpA206K spc divIVA::tet | This work |
| BSN295 | 168 amyE::Pxyl-divIVALm-144-Bs-gfpA206K spc divIVA::tet | This work |
| BSN296 | 168 amyE::Pxyl-divIVALm-130-Bs-gfpA206K spc divIVA::tet | This work |
| BSN297 | 168 amyE::Pxyl-divIVALm-83-Bs-gfpA206K spc divIVA::tet | This work |
| BSN298 | 168 amyE::Pxyl-divIVALm-104-Bs-gfpA206K spc divIVA::tet | This work |
| BSN308 | 168 aprE::Pspac-minJ-gfp cat lacI | This work |
| BSN313 | 168 aprE::Pspac-divIVAR131A spc lacI divIVA::tet | This work |
| BSN316 | 168 amyE::Pxyl-divIVALm-71-Bsspc divIVA::tet | This work |
| BSN317 | 168 aprE::Pspac-minJ-gfp cat | This work |
| BSN321 | 168 amyE::Pxyl-divIVABs-57-Lmspc divIVA::tet | This work |
| BSN332 | 168 aprE::PdivIVA3-minJ-gfp cat | This work |
| BSN333 | 168 aprE::PdivIVA3-minJ-gfp cat divIVA::tet | This work |
| BSN334 | 168 aprE::PdivIVA3-minJ-gfp cat amyE::Pxyl-divIVABsspc divIVA::tet | This work |
| BSN335 | 168 aprE::PdivIVA3-minJ-gfp cat amyE::Pxyl-divIVALmspc divIVA::tet | This work |
| BSN336 | 168 aprE::PdivIVA3-minJ-gfp cat amyE::Pxyl-divIVALm-104-Bsspc divIVA::tet | This work |
| BSN338 | 168 aprE::PdivIVA3-minJ-gfp cat amyE::Pxyl-divIVABs-57-Lmspc divIVA::tet | This work |
| BSN340 | 168 racA::pSG4916(Pxyl-gfp-racA′ cat)amyE::Pxyl-divIVABsspc divIVA::tet | This work |
| BSN341 | 168 racA::pSG4916(Pxyl-gfp-racA′ cat)amyE::Pxyl-divIVALmspc divIVA::tet | This work |
| BSN342 | 168 racA::pSG4916(Pxyl-gfp-racA′ cat)amyE::Pxyl-divIVALm-104-Bsspc divIVA::tet | This work |
| BSN344 | 168 racA::pSG4916(Pxyl-gfp-racA′ cat)amyE::Pxyl-divIVABs-57-Lmspc divIVA::tet | This work |
| BSN356 | 168 aprE::Pspac-divIVABscat lacI divIVA::tet | This work |
| BSN357 | 168 aprE::Pspac-divIVABsR102K cat lacI divIVA::tet | This work |
| BSN358 | 168 aprE::Pspac-divIVABsR102E cat lacI divIVA::tet | This work |
| BSN360 | 168 aprE::Pspac-divIVABsΔC34 cat lacI divIVA::tet | This work |
| BNS372 | 168 amyE::Pxyl-divIVABs-57-Lm-gfpA206K spc divIVA::tet | This work |
| BSN373 | 168 amyE::Pxyl-divIVALm-gfpA206K spc divIVA::tet | This work |
a
The designations represent, e.g., for divIVA1-459(Δ11), divIVA from nucleotide positions 1 to 459 with a deletion of the 11 C-terminal amino acid residues.