Fig 3.
Effect of V37G mutation of SurA stability. (A) Limited trypsin digestion of purified WT SurA and SurAV37G. The molecular masses of bands in the molecular mass marker (M) are marked on the left of the gel. (B) SurA expression levels in different strains. Western blot analysis of osmotic fluid from BW25113, BW25113ΔsurA, and BW25113ΔsurA containing plasmid-encoded WT SurA, SurAV37G, and SurAV37G/A30C/I425C (SurAV37G CC). An anti-SurA antibody was used. The molecular masses of bands in the molecular mass marker are marked on the left of the gels.