Table 1.

Novobiocin MIC of BW25113ΔsurA expressing surA mutants encoded by plasmids

Protein	MIC (μg/ml)	CSc	Mutated residue(s)	MIC (μg/ml)	CS
BW25113a	128		N403A	128	6
pMal-pIIIb	16		K405A	128	7
Wild-type SurA	128		F406A	128	2
V32G	64	5	E408A	64	5
V37G	16	7	W413G	128	7
D41K	128	5	Q415A	128	5
V42A	128	7	E416A	128	2
M114G	128	4	R418G	128	8
Y120A	128	3	Y422A	128	4
Y125A	128	3	I425A	128	4
Y128A	128	5	Y120A/Y125A/Y128A	128
Q131G	128	3	A30C/I425C	256
I132G	128	4	V37G/A30C/I425C	256
E135G	128	6	V89C/I137C	128
M136G	128	9	V42C/I70C	128
Y398A	128	2	V146C/A410C	128

^aThe parent strain is shown as a positive control.

^bBW25113ΔsurA containing vector pMal-pIII is shown as the negative control.

^cCS, conservation score (20).