Table 1.
Primers used in this study
| No. | Name | Sequence (5′→3′)a | Target | Use |
|---|---|---|---|---|
| 1 | pGh9 02 Eco | GGTATACTACTGACAGCTTCC | pGhost9 ISS1 | Sequencing |
| 2 | 5′ ISS1(rev) Eco | CGTAGATAATAACCAACAGCG | pGhost9 ISS1 | Sequencing |
| 3 | up-sagA2-inv-Bw | TTGAGTTGTTTCTGCCACGC | sagA2 | Sequencing |
| 4 | sagB-inv-Bw | AGTCCTTCAACCTGTCTGGC | sagB | Sequencing |
| 5 | sagC-Fw1 | ATGAAATATCAATTAAATAG | sagC | Sequencing |
| 6 | sagC-inv-Bw | ATTGTTCGCCCAGCCTCTGC | sagC | Sequencing |
| 7 | sagC-Fw2 | CACAAGGTGCGCTGGCAAAA | sagC | Sequencing |
| 8 | sagC-Fw3 | CCCGTGAAATGGTAAAAGAC | sagC | Sequencing |
| 9 | sagD-inv-Bw | AAAATCCCTGTCCGATTGCC | sagD | Sequencing |
| 10 | sagD-Fw | CCAATTGATATGACCATCGG | sagD | Sequencing |
| 11 | sagE-Fw | TATTGATGTTGTGCTGACGC | sagE | Sequencing |
| 12 | sagF-Fw | TTTGGTGTATGGTAGCCACC | sagF | Sequencing |
| 13 | sagH-Bw | GCTCCTTCAAGCTGATAGCG | sagH | Sequencing |
| 14 | sagH-Bw2 | CCTAAGCCTAGATTAACGAG | sagH | Sequencing |
| 15 | sagI-down-Bw1 | CAGCTGGCTGCTTTGTTGGC | sagI | Sequencing |
| 16 | sagI-inv-Fw | AAGGGAGTTTATATGCTTCC | sagI-down | Sequencing |
| 17 | sagI-down-Bw2 | GGCACTATTAAAAATAGCAT | sagI-down | Sequencing |
| 18 | sagI-down-Bw3 | GCTACAGACTGTTGACGAAC | sagI-down | Sequencing |
| 19 | sagA1-Fw | ATGTTAAAATTTTCTTCAAACG | sagA1 | Detection |
| 20 | sagA1-Bw | TTATTTTGTAGGTGCTACGG | sagA1 | Detection |
| 21 | sagA2-Fw | ATGCTTAAATTAGATTCACATATTATGG | sagA2 | Detection |
| 22 | sagA2-Bw | TTAAGGTTTAATGTTTGTTGAACC | sagA2 | Detection |
| 23 | up-operon-Fw1 | GACGAGCAGATGTAGATGCC | up-operon | Mutant construction |
| 24 | up-sagA2-fus-Fw | ATGAAAAATTATGAGAATGC | up-sagA2 | Mutant construction |
| 25 | erm-Fw(BamHI) | AATGGATCCCCCGATAGCTTCCGCTATTG | erm | Mutant construction |
| 26 | erm-Bw(PstI) | GTACTGCAGCTAATAATTTATCTACATTCC | erm | Mutant construction |
| 27 | erm-Fw(SalI) | CGCGTCGACCCCGATAGCTTCCGCTATTG | erm | Mutant construction |
| 28 | up-operon-Fw2 | TGTCGCACGGAACCATTCGC | up-operon | Mutant construction |
| 29 | up-operon-Bw(PstI) | GCGCTGCAGGCGCGTTCTTATAGCATTTG | up-operon | Mutant construction |
| 30 | up-operon-Fw(SalI) | CGCGTCGACGATGGTTATATTTGTGAAATAGG | up-operon | Mutant construction |
| 31 | up-operon-Bw | GCGTAAACATTTTCAAACTAC | up-operon | Mutant construction |
| 32 | up-sagA2-Fw(BamHI) | CAGGATCCTCATGGATTGCTAGC | up-sagA2 | Mutant construction |
| 33 | up-sagB-Fw | AATTTTGTTTATAGGAATTAG | up-sagB | Mutant construction |
| 34 | up-sagB-fus-Bw | GCATTCTCATAATTTTTCATC | up-sagB | Mutant construction |
| 35 | sagA1-comp-Fw1(EcoRI) | GGGAATTCGGATTTGATAGTAATGTACG | up-sagA1 | Complementation |
| 36 | sagA1-comp-Fw2(EcoRI) | GGGAATTCAGTGTAAAGATTAGCTAGCC | up-sagA1 | Complementation |
| 37 | sagA1-comp-Bw(PstI) | GCGCTGCAGGCTTCATCCAAAGAATCGTC | sagA1-down | Complementation |
| 38 | sagA2-comp-Fw(EcoRI) | GGGAATTCGTTCATGATGAGTTAAAAAC | up-sagA2 | Complementation |
| 39 | sagA2-comp-Bw(PstI) | GCGCTGCAGAGATGAATCGATGCTTTACC | sagA2-down | Complementation |
| 40 | sagB-S1-Fw | AGCTTGTTGCTCAGCATCTCATGCC | sagB | 5′-RACE |
| 41 | sagB-A1-Bw | GATGATACTCTGAGAAAGAGAAGTGGTTGG | sagB | 5′-RACE |
| 42 | sagB-S2-Fw | TTCTGAATTTTTCACAGATTCAGCTGTAGC | sagB | 5′-RACE |
| 43 | sagB-A2-Bw | GCTTCGTCTCTTGTCAGGTGAGAGG | sagB | 5′-RACE |
| 44 | sagB-RT-Bw(phospho) | Phospho-AGACAGAGAAGCTGC | sagB | 5′-RACE |
| 45 | M13 Forward primer | GTAAAACGACGGCCAGT | pUC18 | Sequencing |
| 46 | M13 Reverse primer | CAGGAAACAGCTATGAC | pUC18 | Sequencing |
a
Restriction sites are underlined.