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All FlhF mutants support the assembly of polar flagella. Bacteria were grown to an OD600 of ∼0.5 in LB plus 0.2% arabinose, fixed, and labeled with anti-FliC conjugated to Alex Fluor 488 as described in Materials and Methods to visualize flagella. (A) PAK; (B) PAK ΔflhF; (C) PAK ΔflhF + WT; (D) PAK ΔflhF + R251G; (E) PAK ΔflhF + L298R,P299L; (F) PAK ΔflhF + K222A; (G) PAK ΔflhF + D294A.
