Table 2.

Primers used in this study

Primer	Sequence (5′–3′)
KA-1	GAA TTC AGG AGG TTC GGG CAA TGC AAG TCA A
KA-2	GAG CTC TCA GTG GTG GTG GTG GTG GTG ACG CCG CGC CGG TTG CTG
R251G-1	GCA TGG ACA GCT ACG GTA TCG GCG CCC AGG
R251G-2	CCT GGG CGC CGA TAC CGT AGC TGT CCA TGC
K222A-1	GCC CGG CAG GTG CCG GAG CGA CCA CCA CCC
K222A-2	GGG TGG TGG TCG CTC CGG CAC CTG CCG GGC
D294A-1	TGG TGC TGA TCG CTA CCG CCG GCC TGC
D294A-2	GCA GGC CGG CGG TAG CGA TCA GCA CCA
L298R,P299L-1	ATA CCG CCG GCC GGC TGG CAA GCG ACC
L298R,P299L-2	GGT CGC TTG CCA GCC GGC CGG CGG TAT
NdeI-flhF-forw	CAT ATG CAA GTC AAA CGC TTC TTC GCC GCC GAT ATG CGC
NotI-flhF-rev	GCG GCC GCG CCG GCA CGC CGC GCC GGT TGC TGG TAG AG